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JoVE Journal Cancer Research
Genome-wide RNAi Screening at identificere vært faktorer, at modulerer Oncolytic Virus terapi
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Genome-wide RNAi Screening at identificere vært faktorer, at modulerer Oncolytic Virus terapi

Article DOI: 10.3791/56913-v • 08:51 min • April 3rd, 2018
April 3rd, 2018 •
1,2, 1,4, 1, 1, 1,2,3
1Children's Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology, University of Ottawa, 3Department of Pediatrics, University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary

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Her beskriver vi en protokol for at ansætte høj overførselshastighed RNAi screening at afdække vært mål, der kan manipuleres til at forbedre oncolytic virus terapi, specielt rhabodvirus og vaccinia virus behandling, men det kan være let tilpasses til andre oncolytic virus platforme eller for at opdage vært gener, at modulere virus replikering generelt.

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Kræftforskning spørgsmål 134 Oncolytic virus RNAi skærm høj overførselshastighed kræft vaccinia rhabdovirus celle vært funktionel genomforskning
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