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JoVE Journal Genetics
En protokol til produktion af Integrase-mangelfuld Lentiviral vektorer for CRISPR/Cas9-medieret gen Knockout i dividere celler
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En protokol til produktion af Integrase-mangelfuld Lentiviral vektorer for CRISPR/Cas9-medieret gen Knockout i dividere celler

Article DOI: 10.3791/56915-v • 10:42 min • December 12th, 2017
December 12th, 2017 •
1, 1
1Duke Viral Vector Core, Department of Neurobiology, Duke University School of Medicine

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Vi beskrive produktion strategien integrase-mangelfuld lentiviral vektorer (IDLVs) som køretøjer til at levere CRISPR/Cas9 til cellerne. Med en evne til at mægle, hurtig og robust gen redigering i celler, IDLVs præsentere et sikrere og lige så effektive vektor platform for gen levering i forhold til integrase-kompetente vektorer.

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Genetik spørgsmålet 130 Viral-medieret genoverførsel HEK-293T celler integrase-mangelfuld lentiviral vektorer CRISPR/Cas9 gen redigering system p24 ELISA saccharose-gradient ultracentrifugering FACS
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