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Neuroscience

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JoVE Journal Neuroscience
Neuron-makrofag Co kulturer at aktivere makrofager secernerende molekylære faktorer med Neurite udvækst aktivitet
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Neuron-makrofag Co kulturer at aktivere makrofager secernerende molekylære faktorer med Neurite udvækst aktivitet

Article DOI: 10.3791/56920 • 08:52 min • March 30th, 2018
March 30th, 2018 •
1,2, 1,2, 1,2,3
1Department of Brain Science, Ajou University School of Medicine, 2Neuroscience Graduate Program, Department of Biomedical Sciences, Ajou University School of Medicine, 3Department of Neurology, Ajou University School of Medicine

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Den nuværende protokol præsenterer eksperimentelle procedurer for at stimulere kulturperler makrofager at være begavet med evnen til at frigive molekylære faktorer, der fremmer neurite udvækst. Behandling af cAMP til neuron-makrofag Co kulturer inducerer makrofager at producere konditioneret medium, der besidder stærke neurite udvækst aktivitet.

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Neurovidenskab sag 133 makrofag dorsalrodsganglier neuron fælles kultur axon regenerering neuron-makrofag interaktion cyklisk AMP konditionering skade
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