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Imaging tilgange til vurderinger af toksikologiske oxidativt Stress ved hjælp af genetisk kodet Fluorogenic sensorer
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Imaging tilgange til vurderinger af toksikologiske oxidativt Stress ved hjælp af genetisk kodet Fluorogenic sensorer

Article DOI: 10.3791/56945-v • 09:33 min • February 7th, 2018
February 7th, 2018 •
1, 2, 2,3
1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education

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Dette manuskript beskriver brugen af genetisk kodet fluorogenic journalister i et program af live-celle imaging til gennemgang af xenobiotiske-induceret oxidativt stress. Denne eksperimentelle tilgang tilbyder uovertruffen spatiotemporelle opløsning, følsomhed og specificitet samtidig undgå mange af manglerne ved konventionelle metoder, der anvendes til påvisning af toksikologiske oxidativt stress.

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Kræftforskning sag 132 oxidativ stress toksikologi fluorescens Live-celle imaging glutathion Hydrogen Peroxid
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