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Journal / Biochemistry / 结合 x 射线晶体学与小角度 x 射线散射到模型的非结构化区域的 Nsa1 从s…
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Biochemistry

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JoVE Journal Biochemistry
结合 x 射线晶体学与小角度 x 射线散射到模型的非结构化区域的 Nsa1 从s 酿酒酵母
Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
Journal (Biochemistry)
Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
Detection of Detergent-sensitive Interactions Between Membrane Proteins
Journal (Biochemistry)
Detection of Detergent-sensitive Interactions Between Membrane Proteins
 
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结合 x 射线晶体学与小角度 x 射线散射到模型的非结构化区域的 Nsa1 从s 酿酒酵母

Article DOI: 10.3791/56953-v • 09:15 min • January 10th, 2018
January 10th, 2018 •
1, 1, 1
1Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

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该方法描述了用 x 射线晶体学和小角 x 射线散射 (SAXS) 进行结构测定的重组 Nsa1 的克隆、表达和纯化, 适用于其他蛋白质的混合结构分析包含有序和无序的域。

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生物化学 问题 131 x-射线结晶学 SAXS 杂交方法 蛋白质纯化 WD40 领域 有限的蛋白质水解 核糖体汇编
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