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Journal / Biochemistry / Kombinere Røntgenkrystallografi med liten vinkel…
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Biochemistry

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JoVE Journal Biochemistry
Kombinere Røntgenkrystallografi med liten vinkel X-Ray spredning modell ustrukturert regioner i Nsa1 fra S. Cerevisiae
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
Journal (Biochemistry)
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
Locus-Specific Chromatin Purification from Saccharomyces cerevisiae
Journal (Biochemistry)
Locus-Specific Chromatin Purification from Saccharomyces cerevisiae
 
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Kombinere Røntgenkrystallografi med liten vinkel X-Ray spredning modell ustrukturert regioner i Nsa1 fra S. Cerevisiae

Article DOI: 10.3791/56953-v • 09:15 min • January 10th, 2018
January 10th, 2018 •
1, 1, 1
1Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

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Denne metoden beskriver kloning, uttrykk og rensing av rekombinant Nsa1 for strukturelle vilje av Røntgenkrystallografi og liten vinkel X-ray spredning (SAXS) og gjelder for hybrid strukturell analyse av andre proteiner som inneholder begge bestilte og uordnede domener.

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Biokjemi problemet 131 Røntgenkrystallografi SAXS Hybrid metoder Protein rensing WD40 domene begrenset proteolyse ribosom montering
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