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Mikrosonde Capillary Electrophoresis Mass Spectrometry für einzellige Metabolomik in Live-Frosch (Xenopus Laevis) Embryonen
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Mikrosonde Capillary Electrophoresis Mass Spectrometry für einzellige Metabolomik in Live-Frosch (Xenopus Laevis) Embryonen

Article DOI: 10.3791/56956-v • 12:16 min • December 22nd, 2017
December 22nd, 2017 •
1, 1, 2, 1,3
1Department of Chemistry, George Washington University, 2Department of Anatomy & Regenerative Biology, George Washington University, 3Department of Chemistry & Biochemistry, University of Maryland, College Park

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Wir beschreiben Schritte, mit denen schnell in Situ Probenahme von einen kleinen Teil einer einzelnen Zelle mit hoher Präzision und minimalen Invasion mit Kapillar-basierten Mikro-Probenahme, chemische Charakterisierung einer Momentaufnahme der metabolischen Aktivität in zu erleichtern Embryonen mit einer speziell angefertigten Einzelzelle Kapillarelektrophorese und Massenspektrometrie-Plattform zu leben.

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Chemie Ausgabe 130 Kapillar-Elektrophorese Elektrospray-Ionisation Massenspektrometrie Microsampling Metabolomik Einzelzelle Xenopus Frosch Embryogenese
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