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Journal / Developmental Biology / Misura in tempo reale di permeabilità della…
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Developmental Biology

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JoVE Journal Developmental Biology
Misura in tempo reale di permeabilità della barriera epiteliale in Organoids intestinale umana
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Misura in tempo reale di permeabilità della barriera epiteliale in Organoids intestinale umana

Article DOI: 10.3791/56960-v • 08:04 min • December 18th, 2017
December 18th, 2017 •
1, 1, 1, 1,2, 1,3
1Department of Internal Medicine, Division of Gastroenterology, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease, University of Michigan

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Questo protocollo descrive la misura della permeabilità della barriera epiteliale in tempo reale dopo il trattamento farmacologico in organoids intestinale umana utilizzando la microscopia a fluorescenza e microscopia cellulare di vivere.

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Biologia dello sviluppo problema 130 organoids intestinale umano microiniezione permeabilità della barriera epiteliale enteroids coltura del tessuto 3D imaging in vitro
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