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JoVE Journal Cancer Research
Flow flowcytometri for at vurdere leukæmi stamceller i primære akut Myeloid leukæmi og Patient-afledt-xenografts, på diagnose og følg op
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Flow flowcytometri for at vurdere leukæmi stamceller i primære akut Myeloid leukæmi og Patient-afledt-xenografts, på diagnose og følg op

Article DOI: 10.3791/56976-v • 09:01 min • March 26th, 2018
March 26th, 2018 •
1,2, 2,3, 4, 2, 2, 2, 2,5, 1,2, 1,2, 2
1Laboratoire d’hématologie, CHU Lille, 2Univ. Lille, Inserm, CHU Lille, UMR-S 1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, 3Service d’hématologie pédiatrique, hôpital Jeanne de Flandre, CHU Lille, 4Laboratoire d’hématologie CH Lyon Sud, 5Service des Maladies du Sang, hôpital Claude Huriez, CHU Lille

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Vi skitsere en protokol for at opdage samtidig udtryk for leukæmi stamceller markører på primære akut myeloid leukæmiceller ved flowcytometri. Vi viser hvordan man kan kvantificere tre stamfader befolkninger og en formodede LSC befolkningen med stigende grad af modning. Vi bekræftet tilstedeværelsen af disse befolkningsgrupper i tilsvarende patient-afledt-xenografts.

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Kræftforskning sag 133 akut myeloid leukæmi AML leukæmi stamceller LSC bloddannelsen patient-afledt-xenografts PDX multiparametric flowcytometri minimal residual sygdom MRD
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