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Interactome-Seq: En protokol til Domainome bibliotek konstruktion, validering og udvælgelse af Phage Display og næste Generation Sequencing

Article DOI: 10.3791/56981-v • 12:04 min • October 3rd, 2018
October 3rd, 2018 •
1, 2, 2, 3, 3, 3, 4, 1, 4, 5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy

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De protokoller er beskrevet tillader opbygning, karakterisering og udvælgelse (mod mål på valg) af en "domainome" bibliotek fra DNA-kilder. Dette opnås ved en forskning rørledning, der kombinerer forskellige teknologier: phage display, en falsning reporter og næste generation sequencing med en web-værktøj til dataanalyse.

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Biologi spørgsmål 140 Phage display næste Generation Sequencing Interactome Protein domæne web-værktøj folde reporter protein struktur.
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