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Journal / Biology / Interactome-Seq: Ett protokoll för Domainome…
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Interactome-Seq
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Journal (Biology)
Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Journal (Biology)
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Interactome-Seq: Ett protokoll för Domainome bibliotek konstruktion, validering och urval av Phage Display och nästa generations sekvensering

Article DOI: 10.3791/56981-v • 12:04 min • October 3rd, 2018
October 3rd, 2018 •
1, 2, 2, 3, 3, 3, 4, 1, 4, 5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy

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De protokoll som beskrivs kan konstruktion, karakterisering och urval (mot målet för val) av ett ”domainome” bibliotek från någon DNA-källa. Detta uppnås genom en forskning pipeline som kombinerar olika tekniker: phage display, fällbara reporter och nästa generations sekvensering med en webb-verktyg för dataanalys.

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Biologi fråga 140 Phage display nästa generations sekvensering Interactome Protein domän webbverktyg fällbara reporter proteinstruktur.
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