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Journal / Biology / Interactome-Seq: Um protocolo para a construção…
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JoVE Journal Biology
Interactome-Seq
A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines
Journal (Biology)
A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines
Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays
Journal (Biology)
Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays
 
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Interactome-Seq: Um protocolo para a construção da biblioteca de Domainome, a validação e a seleção por Phage Display e a próxima geração de sequenciamento

Article DOI: 10.3791/56981-v • 12:04 min • October 3rd, 2018
October 3rd, 2018 •
1, 2, 2, 3, 3, 3, 4, 1, 4, 5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy

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Os protocolos descritos permitem a construção, caracterização e seleção (contra o destino de escolha), de uma biblioteca de "domainome", feita a partir de qualquer fonte de DNA. Isto é conseguido por um pipeline de pesquisa que combina diferentes tecnologias: a exibição do fago, repórter dobrável e sequenciamento de próxima geração com uma ferramenta web para análise de dados.

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Biologia questão 140 Phage exibir na próxima geração de sequenciamento Interactome domínio da proteína ferramenta web repórter dobrável estrutura da proteína.
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