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Journal / Biochemistry / Rensning af hepatocytter og sinusformet…
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Biochemistry

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JoVE Journal Biochemistry
Rensning af hepatocytter og sinusformet endotelceller fra mus lever Perfusion
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Rensning af hepatocytter og sinusformet endotelceller fra mus lever Perfusion

Article DOI: 10.3791/56993-v • 08:54 min • February 12th, 2018
February 12th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Biochemistry, University of Nebraska

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Målet med denne protokol er at opnå høj rentabilitet og højt udbytte af hepatocytter og sinusformet endotelceller fra leveren. Dette opnås ved perfusing lever med en type IV collagenase løsning via portalen vene, efterfulgt af differential centrifugering hepatocytter og sinusformet endotelceller.

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Biokemi sag 132 leveren perfusion hepatocyt sinusformet endotelceller mus lever murine leveren collagenase celle rensning
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