Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / 小鼠肝灌注肝细胞和正弦内皮细胞的纯化…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
小鼠肝灌注肝细胞和正弦内皮细胞的纯化
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Journal (Biochemistry)
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Manually Extracting the Bradford Assay Data Recorded with a Smartphone (Video) | JoVE
Journal (Biochemistry)
Manually Extracting the Bradford Assay Data Recorded with a Smartphone (Video) | JoVE
 
Click here for the English version

小鼠肝灌注肝细胞和正弦内皮细胞的纯化

Article DOI: 10.3791/56993-v • 08:54 min • February 12th, 2018
February 12th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Biochemistry, University of Nebraska

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

该协议的目的是获得高生存能力和高产量的肝细胞和正弦内皮细胞。这是通过灌注肝与 IV 型胶原酶溶液通过门静脉, 其次是差异离心获得肝细胞和正弦内皮细胞。

Tags

生物化学 问题 132 肝脏灌注 肝细胞 正弦内皮细胞 小鼠肝脏 鼠肝 胶原酶 细胞纯化
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter