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Journal / Biochemistry / Zuivering van hepatocyten en sinusvormige…
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Biochemistry

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JoVE Journal Biochemistry
Zuivering van hepatocyten en sinusvormige endotheliale cellen van lever muis perfusie
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Zuivering van hepatocyten en sinusvormige endotheliale cellen van lever muis perfusie

Article DOI: 10.3791/56993-v • 08:54 min • February 12th, 2018
February 12th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Biochemistry, University of Nebraska

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Het doel van dit protocol is te verkrijgen van hoge levensvatbaarheid en hoog rendement van hepatocyten en sinusoïdale endotheliale cellen van lever. Dit wordt bereikt door zoogdierlevercellen de lever met een type IV collagenase oplossing via de portal ader, gevolgd door differentiële centrifugeren hepatocyten en sinusoïdale endotheliale cellen te verkrijgen.

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Biochemie kwestie 132 lever perfusie hepatocyte sinusvormige endotheliale cellen muis lever lymfkliertest lever collagenase cel zuivering
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