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Journal / Immunology and Infection / İki fotonlu lazer mikroskop kullanarak hücre zarı …
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Immunology and Infection

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JoVE Journal Immunology and Infection
İki fotonlu lazer mikroskop kullanarak hücre zarı tamir tahlil
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İki fotonlu lazer mikroskop kullanarak hücre zarı tamir tahlil

Article DOI: 10.3791/56999-v • 06:35 min • January 2nd, 2018
January 2nd, 2018 •
1, 1, 2, 1
1Department of Medical Genetics, University of Alberta Faculty of Medicine and Dentistry, 2Center for iPS Cell Research and Application, Kyoto University

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Hücre zarının iki fotonlu lazer ile yaralama membran yeniden mühürlemeden yeteneği değerlendirmek için yaygın olarak kullanılan bir yöntemdir ve birden çok hücre türü için uygulanabilir. Burada, vitro canlı İki fotonlu lazer ablasyon takip dysferlinopathy hasta hücrelerde membran yeniden mühürleme, görüntüleme için bir protokol açıklayın.

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İmmünoloji sayı: 131 iki-foton/multi-photon lazer mikroskobu membran onarım hücre plazma zarı dysferlin mitsugumin-53 annexin lazer ablasyon/yaralama vitro canlı görüntüleme dysferlinopathy bacak-korse kas distrofisi türü IIB anterior tibial başlangıçlı Miyoshi myopathy ile distal myopathy
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