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Standardiserad mätning av näsans membran Transepithelial Potential skillnad (NPD)
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Standardiserad mätning av näsans membran Transepithelial Potential skillnad (NPD)

Article DOI: 10.3791/57006-v • 09:47 min • September 13th, 2018
September 13th, 2018 •
1, 2, 3, 4, 5, 1, 6,7
1Department of Medicine and the Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, 2Department of Pulmonology and Tuberculosis, University Medical Center Utrecht, 3Center for Experimental Medicine, Queens University, Northern Ireland, 4Hadassah Hebrew University Medical Center, Jerusalem, 5Centro Fibrosi Cistica, Azienda Ospedaliera Universitaria Integrata, 6Service de Pneumologie et Allergologie Pédiatriques and Center de Ressources et de Compétence de la Mucoviscidose, Hôpital Necker Enfants Malades, 7INSERM U 1151, Institut Necker Enfants Malades

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Här presenterar vi ett standardiserat protokoll för att mäta nasal potentialskillnaden (NPD). Cystisk fibros transmembrane conductance regulator (CFTR) och epitelial natrium kanal (ENaC) funktion utvärderas av förändringen i spänningen över nasal epitelet efter superfusion av lösningar som ändra ion kanal aktivitet, som ger en effektmåttet.

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Medicin fråga 139 Nasal potentialskillnad cystisk fibros cystisk fibros Transmembrane Conductance Regulator CFTR
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