Summary
September 13th, 2018
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Aquí, presentamos un protocolo estandarizado para medir la diferencia de potencial nasal (NPD). Regulador de la conductancia transmembrana de la fibrosis quística (CFTR) y función epitelial del sodio (ENaC) son evaluados por el cambio en el voltaje a través del epitelio nasal después de la superfusion de soluciones que modifican la actividad del canal del ion, proporcionando un medida de resultado.
Transcript
This method can help answer key questions in the CF and ion transport fields about whether CF patients can mount a meaningful response to activation or restoration of CFTR function.The main advantage of this technique is that it measures the coordinated activity of the CFTR and epithelial sodium channel in the airway epithelium.Although this method can provide insight into cystic fibrosis, it can also be applied to other disease systems, such as genetic disorders of hyperactive epithelial sodium channel function.Demonstrating the procedure will be Thao Nguyen, biologist, and Aur