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Medición estandarizada de la diferencia de potencial transepitelial Nasal membrana (NPD)
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Medición estandarizada de la diferencia de potencial transepitelial Nasal membrana (NPD)

Article DOI: 10.3791/57006-v • 09:47 min • September 13th, 2018
September 13th, 2018 •
1, 2, 3, 4, 5, 1, 6,7
1Department of Medicine and the Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, 2Department of Pulmonology and Tuberculosis, University Medical Center Utrecht, 3Center for Experimental Medicine, Queens University, Northern Ireland, 4Hadassah Hebrew University Medical Center, Jerusalem, 5Centro Fibrosi Cistica, Azienda Ospedaliera Universitaria Integrata, 6Service de Pneumologie et Allergologie Pédiatriques and Center de Ressources et de Compétence de la Mucoviscidose, Hôpital Necker Enfants Malades, 7INSERM U 1151, Institut Necker Enfants Malades

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Aquí, presentamos un protocolo estandarizado para medir la diferencia de potencial nasal (NPD). Regulador de la conductancia transmembrana de la fibrosis quística (CFTR) y función epitelial del sodio (ENaC) son evaluados por el cambio en el voltaje a través del epitelio nasal después de la superfusion de soluciones que modifican la actividad del canal del ion, proporcionando un medida de resultado.

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Medicina número 139 diferencia de potencial Nasal Fibrosis quística regulador de la conductancia transmembrana de la Fibrosis quística CFTR
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