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Journal / Cancer Research / Isolering og karakterisering af tumor initierende …
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Cancer Research

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JoVE Journal Cancer Research
Isolering og karakterisering af tumor initierende celler fra sarkom patient afledte Xenografts
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Isolering og karakterisering af tumor initierende celler fra sarkom patient afledte Xenografts

Article DOI: 10.3791/57011-v • 07:18 min • June 13th, 2019
June 13th, 2019 •
1, 1, 1, 1, 1
1Department of Pathology, Icahn School of Medicine at Mount Sinai

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Vi beskriver en detaljeret protokol for isolering af tumor initierende celler fra humane sarkom patient-afledte xenografter ved fluorescens-aktiveret celle sortering, ved hjælp af humant leukocytantigen-1 (HLA-1) som en negativ markør, og for yderligere validering og karakterisering af disse HLA-1-negative tumor-initierende celler.

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Kræftforskning tumor initierende celler patient-afledte xenograft humant leukocytantigen-1 sarcomas humane vævsprøver intratumoral heterogenitet
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