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JoVE Journal
Cancer Research

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来自沙柯玛患者衍生异种移植物的肿瘤启动细胞的分离和表征
 
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来自沙柯玛患者衍生异种移植物的肿瘤启动细胞的分离和表征

Article DOI: 10.3791/57011
June 13th, 2019

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Summary June 13th, 2019

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我们描述了通过荧光活性细胞分类,使用人类白细胞抗原-1(HLA-1)作为阴性标记,从人类肉瘤患者衍生的异种移植物分离肿瘤启动细胞的详细方案,以及进一步的验证和这些HLA-1阴性肿瘤启动细胞的表征。

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