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JoVE Journal
Cancer Research

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Isolering og karakterisering af tumor initierende celler fra sarkom patient afledte Xenografts
 
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Isolering og karakterisering af tumor initierende celler fra sarkom patient afledte Xenografts

Article DOI: 10.3791/57011
June 13th, 2019

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Summary June 13th, 2019

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Click here for the English version.

Vi beskriver en detaljeret protokol for isolering af tumor initierende celler fra humane sarkom patient-afledte xenografter ved fluorescens-aktiveret celle sortering, ved hjælp af humant leukocytantigen-1 (HLA-1) som en negativ markør, og for yderligere validering og karakterisering af disse HLA-1-negative tumor-initierende celler.

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