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Journal / Cancer Research / Isolering och karakterisering av tumör-initiera…
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Cancer Research

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JoVE Journal Cancer Research
Isolering och karakterisering av tumör-initiera celler från sarkom patient-derived xenografts
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Isolering och karakterisering av tumör-initiera celler från sarkom patient-derived xenografts

Article DOI: 10.3791/57011-v • 07:18 min • June 13th, 2019
June 13th, 2019 •
1, 1, 1, 1, 1
1Department of Pathology, Icahn School of Medicine at Mount Sinai

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Vi beskriver ett detaljerat protokoll för isolering av tumör-initierande celler från humana sarkom-patientbaserade xenografter genom fluorescensaktiverad cell sortering, med humant leukocytantigen-1 (HLA-1) som en negativ markör, och för ytterligare validering och karakteriseringen av dessa HLA-1-negativa tumör-initierande celler.

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Cancer forskning fråga 148 tumör-initierande celler patient-derived xenograft humant leukocyt antigen-1 sarcomas mänskliga vävnadsprover intratumorala heterogenitet
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