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Native Chromatin Immunopräzipitation mit Murine Gehirn Tumor Neurosphären

Article DOI: 10.3791/57016-v • 09:31 min • January 29th, 2018
January 29th, 2018 •
1, 1,2, 3,4, 1,2, 1,2
1Department of Cell and Developmental Biology, University of Michigan Medical School, 2Department of Neurosurgery, University of Michigan Medical School, 3Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program, University of Michigan Medical School, 4Department of Biology, University of Puerto Rico-Río Piedras Campus

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Epigenetische Mechanismen werden häufig in Gliom verändert. Chromatin Immunopräzipitation könnte verwendet werden, um die Auswirkungen der genetischen Veränderungen, die in Gliom zu studieren, die aus Änderungen in Histon Änderungen ergeben, die Chromatin Struktur und gen-Transkription regulieren. Dieses Protokoll beschreibt native Chromatin Immunopräzipitation auf murine Gehirn Tumor Neurosphären.

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Cancer Research Ausgabe 131 Chromatin Immunopräzipitation Epigenetik Gliom Neurosphäre Generation Histon-Modifikationen
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