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Journal / Genetics / Semi-quantitativen Nachweis von RNA-abhängige…
JoVE Journal
Genetics

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JoVE Journal Genetics
Semi-quantitativen Nachweis von RNA-abhängige RNA-Polymerase-Aktivität des menschlichen Telomerase Reverse Transkriptase Protein
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Journal (Genetics)
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
Journal (Genetics)
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
 
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Semi-quantitativen Nachweis von RNA-abhängige RNA-Polymerase-Aktivität des menschlichen Telomerase Reverse Transkriptase Protein

Article DOI: 10.3791/57021-v • 08:26 min • June 12th, 2018
June 12th, 2018 •
*1, *1, 1, 1, 1
1Division of Cancer Stem Cell, National Cancer Center Research Institute
* These authors contributed equally

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Menschliche Telomerase Reverse Transkriptase (TERT) synthetisiert nicht nur telomeric DNA, sondern auch doppelsträngige RNA durch RNA-abhängige RNA-Polymerase-Aktivität. Hier beschreiben wir einen neu gegründeten Assay um RNA-abhängige RNA-Polymerase-Aktivität der endogenen TERT zu erkennen.

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Genetik Ausgabe 136 TERT RNA-abhängige RNA-Polymerase doppelsträngige RNA Telomerase Immunopräzipitation Radioisotopen
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