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In Vitro und In Vivo Ansätze Darm Epithelzelle Durchlässigkeit zu bestimmen
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In Vitro und In Vivo Ansätze Darm Epithelzelle Durchlässigkeit zu bestimmen

Article DOI: 10.3791/57032-v • 10:22 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University
* These authors contributed equally

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Zwei Methoden werden hier vorgestellt, um intestinale Barrierefunktion zu bestimmen. Eine epitheliale Meter (Volt/Ohm) dient zur Messung des elektrischen Widerstands berührt der kultivierten Epithelien direkt in Gewebekultur Brunnen. Bei Mäusen ist die FITC-Dextran-Magensonde-Methode zur Ermittlung der intestinalen Permeabilität in Vivo.

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Biologie Ausgabe 140 intestinale Barrierefunktion intestinale Permeabilität berührt elektrischen Widerstand FITC-Dextran Caco-2BBe Zellen Magensonde
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