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In Vitro en In Vivo benaderingen te bepalen van intestinale epitheliale cel permeabiliteit
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In Vitro en In Vivo benaderingen te bepalen van intestinale epitheliale cel permeabiliteit

Article DOI: 10.3791/57032-v • 10:22 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University
* These authors contributed equally

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Twee methoden worden hier voorgesteld om te bepalen van intestinale barrièrefunctie. Een epitheliale meter (volt/ohm) wordt gebruikt voor metingen van de elektrische weerstand van de transepithelial van gekweekte epithelen rechtstreeks in weefselkweek wells. Bij muizen, wordt de FITC-dextran maagsonde methode gebruikt om te bepalen de intestinale permeabiliteit in vivo.

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Biologie kwestie 140 intestinale barrièrefunctie intestinale permeabiliteit transepithelial elektrische weerstand FITC-dextran Caco-2BBe cellen maagsonde
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