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JoVE Journal Developmental Biology
Proliferação e diferenciação de murino Precursor mieloide 32G/G-CSF-R células
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Proliferação e diferenciação de murino Precursor mieloide 32G/G-CSF-R células

Article DOI: 10.3791/57033-v • 10:21 min • February 21st, 2018
February 21st, 2018 •
*1,2, *1, 1, 1,2
1Department of Hemato-Oncology, Institute of Molecular Genetics of the ASCR, 2Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University
* These authors contributed equally

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Apresentam-se aqui detalhados protocolos para a linha de celular de 32G/G-CSF-R precursor mieloide murino de cultivo, realizando a infecções virais e realizar ensaios de proliferação e diferenciação. Esta linha de celular é adequada para estudar o desenvolvimento de células mieloides e o papel dos genes de interesse no crescimento de células mieloides e diferenciação neutrofílica.

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Biologia do desenvolvimento questão 132 32G/G-CSF-R células células precursoras mieloides murino cultura líquida diferenciação neutrófilos proliferação citocinas IL-3 G-CSF
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