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Journal / Developmental Biology / Multikombinerbare behandling af Trichostatin A og …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Multikombinerbare behandling af Trichostatin A og C-Vitamin forbedrer effektiviteten af kloning mus af kropsceller
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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Multikombinerbare behandling af Trichostatin A og C-Vitamin forbedrer effektiviteten af kloning mus af kropsceller

Article DOI: 10.3791/57036-v • 09:52 min • April 26th, 2018
April 26th, 2018 •
1, 2, 3, 4, 1,5
1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, 2Faculty of Biology-Oriented Science and Technology, Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, 5Institute of Advanced Technology, Kindai University

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Vi beskriver en dramatisk forbedret metode til musen kloning ved hjælp af trichostatin A, vitamin C og deioniseret bovint serumalbumin. Vi viser et forenklet, reproducerbare protokol, der understøtter effektiv udvikling af klonede embryoner. Derfor kunne denne metode blive en standardiseret procedure for musen kloning.

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Udviklingsmæssige biologi spørgsmål 134 kropsceller Histon Deacetylase Inhibitor Trichostatin A Histon H3 lysin 9 Trimethylation Vitamin C deioniseret bovin Serum Albumin mus Hemagglutinating Virus af Japan kuvert
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