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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

Article DOI: 10.3791/57038-v • 09:35 min • February 26th, 2018
February 26th, 2018 •
1,2, 1, 1, 1
1Institute of Neurology (Edinger Institute), Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology, Goethe University Hospital

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Summary
Automatic Translation

Here we present a mouse brain vascular permeability assay using intraperitoneal injection of fluorescent tracers followed by perfusion that is applicable to animal models of blood-brain barrier dysfunction. One hemi-brain is used for assessing permeability quantitatively and the other for tracer visualization/immunostaining. The procedure takes 5 - 6 h for 10 mice.

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In Vivo Blood-brain Barrier Permeability Assay Mice Fluorescently Labeled Tracers Brain Vasculature Blood-brain Barrier Dysfunction Animal Models Neuro-vascular Permeability Diseases Therapy Simple And Quantitative Method Fluorometry Fluorescence Microscopy Regional Defenses Neurological Disorders Brain Tumors Stroke Alzheimer's Disease Brain Edema BBD Function Therapeutic Target Procedure Miss Jadranka Macas Scientific Technology Officer Intraperitoneal Injection Tracer Solution Lysine-fixable Tracers Dextrans Labeled With FITC And TMR Excitation Emission Spectra Sham Controls Autofluorescence
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