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Journal / Immunology and Infection / Avaliação do citotóxicos e efeitos…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Avaliação do citotóxicos e efeitos imunomoduladores de substâncias no pulmão humano de precisão de corte fatias
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Avaliação do citotóxicos e efeitos imunomoduladores de substâncias no pulmão humano de precisão de corte fatias

Article DOI: 10.3791/57042-v • 12:04 min • May 9th, 2018
May 9th, 2018 •
*1, *1, 1, 1, 1, 2, 2, 3, 4, 4, 5, 1,6, 1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
* These authors contributed equally

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Tendo em conta o princípio dos 3R, modelos respiratórios como alternativas para estudos animais estão evoluindo. Especialmente para a avaliação dos riscos das substâncias respiratórias, há uma falta de ensaios adequados. Aqui, descrevemos o uso de fatias de pulmão humano de precisão de corte para a avaliação de substâncias no ar.

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Imunologia e infecção edição 135 cultura humana explante tecido organotypic biópsia material de pulmão ex vivo citotoxicidade imunomodulação microscopia confocal produtos químicos fatias de pulmão de precisão de corte
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