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Journal / Immunology and Infection / Valutazione dei citotossici e gli effetti…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Valutazione dei citotossici e gli effetti immunomodulatori di sostanze in fette di polmone umano di taglio di precisione
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Valutazione dei citotossici e gli effetti immunomodulatori di sostanze in fette di polmone umano di taglio di precisione

Article DOI: 10.3791/57042-v • 12:04 min • May 9th, 2018
May 9th, 2018 •
*1, *1, 1, 1, 1, 2, 2, 3, 4, 4, 5, 1,6, 1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
* These authors contributed equally

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Summary
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In considerazione del principio di 3Rs, modelli respiratori in alternativa agli studi sugli animali si stanno evolvendo. Soprattutto per la valutazione del rischio delle sostanze respiratorie, c'è una mancanza di analisi appropriate. Qui, descriviamo l'uso delle fette di polmone umano di taglio di precisione per la valutazione delle sostanze trasportate dall'aria.

Tags

Immunologia e infezione problema 135 cultura umana explant organotipiche tessuto biopsia materiale del polmone ex vivo citotossicità immunomodulazione microscopia confocale prodotti chimici fette di taglio di precisione del polmone
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