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Journal / Developmental Biology / Analisi cinetica di vasculogenesi quantifica…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Analisi cinetica di vasculogenesi quantifica dinamiche della vasculogenesi e dell'angiogenesi In Vitro
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Analisi cinetica di vasculogenesi quantifica dinamiche della vasculogenesi e dell'angiogenesi In Vitro

Article DOI: 10.3791/57044-v • 11:03 min • January 31st, 2018
January 31st, 2018 •
1,2, 3,4, 3,4, 1,2,5,6,7
1Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 3Indiana Center for Biological Microscopy, Indiana University School of Medicine, 4Department of Medicine, Indiana University School of Medicine, 5Department of Pediatrics, Indiana University School of Medicine, 6Department of Microbiology and Immunology, Indiana University School of Medicine, 7Indiana University Simon Cancer Center, Indiana University School of Medicine

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Qui, presentiamo un protocollo per l'imaging di time-lapse e analisi di vasculogenesi in vitro usando accoppiata con il software open source, analisi cinetica di vasculogenesi di microscopia di contrasto di fase. Questo protocollo può essere applicato per valutare quantitativamente il potenziale vasculogenic di numerosi tipi cellulari o condizioni sperimentali che modellano la malattia vascolare.

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Biologia dello sviluppo problema 131 vasculogenesi colonie endoteliali cellule microscopia diabete gestazionale time-lapse di imaging cinetica formazione di rete
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