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Journal / Developmental Biology / Análisis cinético de vasculogénesis cuantifica la …
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Developmental Biology

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JoVE Journal Developmental Biology
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Análisis cinético de vasculogénesis cuantifica la dinámica de la vasculogénesis y angiogénesis In Vitro

Article DOI: 10.3791/57044-v • 11:03 min • January 31st, 2018
January 31st, 2018 •
1,2, 3,4, 3,4, 1,2,5,6,7
1Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 3Indiana Center for Biological Microscopy, Indiana University School of Medicine, 4Department of Medicine, Indiana University School of Medicine, 5Department of Pediatrics, Indiana University School of Medicine, 6Department of Microbiology and Immunology, Indiana University School of Medicine, 7Indiana University Simon Cancer Center, Indiana University School of Medicine

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Aquí, presentamos un protocolo para la proyección de imagen de Time-lapse y fase de análisis de la vasculogénesis en vitro usando microscopía de contraste junto con el software de código abierto, análisis cinético de la vasculogénesis. Este protocolo se puede aplicar para evaluar cuantitativamente el potencial de Vasculogénica de numerosos tipos de células o condiciones experimentales que modelo de enfermedad vascular.

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Biología del desarrollo número 131 vasculogénesis endoteliales formadoras de las células microscopía diabetes gestacional Time-lapse de imágenes cinética red de formación
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