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En enkel High Efficiency Protocol for bukspyttkjertelen Islet Isolation fra mus
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En enkel High Efficiency Protocol for bukspyttkjertelen Islet Isolation fra mus

Article DOI: 10.3791/57048-v • 06:56 min • August 30th, 2019
August 30th, 2019 •
1, 2, 1,2, 1, 1, 1,2
1Department of Nutrition and Food Science, Texas A&M University, 2Children's Nutrition Research Center, Baylor College of Medicine

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Dette Holme isolasjons protokollen beskrev en roman rute av kollagenase injeksjon å fordøye eksokrin vev og en forenklet gradient prosedyre for å rense holmer fra mus. Det innebærer enzymatisk fordøyelse, gradient separasjon/rensing, og Holme hånd-plukking. Vellykket isolering kan gi 250 – 350 høy kvalitet og fullt funksjonelle holmer per mus.

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Biologi holmer bukspyttkjertel kollagenase tetthet gradient insulin diabetes mus
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