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JoVE Journal
Biochemistry

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基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1
 
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基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1

Article DOI: 10.3791/57051-v 06:18 min March 13th, 2018
March 13th, 2018

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Summary

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目前用于检测 Zika、基孔肯亚和登革热病毒的复用诊断程序需要复杂的样本准备和昂贵的仪器, 而且在低资源环境下很难使用。我们显示一个诊断, 使用等温放大与目标特定的链不可替代探针, 以检测和区分这些病毒具有高灵敏度和特异性。

Tags

生物化学 问题 133 注意点诊断 复用等温放大 回路介导的等温放大 Zika 检测 荧光读出 没有 RNA 提取 蚊子监视 病毒检测
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