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JoVE Journal Genetics
Undersøgelse af RNA syntese ved hjælp af 5-Bromouridine mærkning og Immunoprecipitation
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Undersøgelse af RNA syntese ved hjælp af 5-Bromouridine mærkning og Immunoprecipitation

Article DOI: 10.3791/57056-v • 09:59 min • May 3rd, 2018
May 3rd, 2018 •
1, 1, 2, 2, 1
1DANDRITE - Danish Research Institute of Translational Neuroscience, Dept. of Biomedicine, Aarhus University, 2Dept. of Molecular Biology and Genetics, Aarhus University

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Denne metode kan bruges til at måle RNA syntese. 5-Bromouridine er føjet til celler og indarbejdet i syntetisk RNA. RNA syntese er målt af RNA udvinding umiddelbart efter mærkning, efterfulgt af 5-Bromouridine-målrettet immunoprecipitation af mærket RNA og analyse af reverse transkription og kvantitative polymerase kædereaktion.

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Genetik spørgsmålet 135 RNA syntese 5-Bromouridine mærkning immunoprecipitation fenol-chloroform udvinding reverse transkription kvantitative polymerase kæde reaktion
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