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JoVE Journal Neuroscience
Karakterisering och isolering av mus primära mikroglia täthet lutning centrifugering
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Karakterisering och isolering av mus primära mikroglia täthet lutning centrifugering

Article DOI: 10.3791/57065-v • 10:22 min • February 16th, 2018
February 16th, 2018 •
*1, 1,2, 1, *1
1The Ritchie Centre, Hudson Institute of Medical Research, 2Department of Obstetrics & Gynaecology, Monash University
* These authors contributed equally

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Ett protokoll för isolering av primära mikroglia från murina hjärnor presenteras. Denna teknik hjälper främja aktuell kunskap om neurologiska sjukdomar. Densitet gradient centrifugering och magnetisk separering kombineras för att producera tillräcklig avkastning av ett högt rena urval. Dessutom beskriver vi stegen för karaktärisering av mikroglia.

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Neurovetenskap fråga 132 mikroglia isolering primär cell cellkultur inflammation djurmodell mus neonatal neurovetenskap perinatal hjärnskada
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