00:49Fluorescence Labeling of Extracellular Vesicles
03:05Visualize Uptake of Extracellular Vesicles by Confocal Microscopy
06:18Endothelial Cell Permeability
07:36Determine Puromycin Sensitivity
08:59Transduction of Endothelial Cells with Lentiviral Vector
10:53Results: Use of Confocal Microscopy and Diffusion Capacity of Fluorochrome Dextran to Study the Interaction Between the Extracellular Vesicles and Endothelial Cells
Neste trabalho, descrevemos os protocolos para investigar o papel das vesículas extracelulares (EVs), lançado pelo Plasmodium falciparum infectado hemácias. Em particular, enfocamos as interações de EVs com células endoteliais.