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Journal / Chemistry / 生物哺乳动物细胞光交联图谱和 Bioorthogonal…
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JoVE Journal Chemistry
生物哺乳动物细胞光交联图谱和 Bioorthogonal 化学中化学探针的遗传受体优化
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生物哺乳动物细胞光交联图谱和 Bioorthogonal 化学中化学探针的遗传受体优化

Article DOI: 10.3791/57069-v • 14:02 min • April 9th, 2018
April 9th, 2018 •
*1, *1, *1, 1
1Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig
* These authors contributed equally

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本文提出了一种简便的荧光分析方法, 用于评价将非规范氨基酸 (ncAAs) 纳入哺乳动物细胞表达的蛋白质中的氨基酰 tRNA 合成酶/tRNA 对的效率。介绍了 ncAAs 在 G 蛋白耦合受体 (受体) 研究中的应用, 包括结合位点的光交联映射和活细胞的 bioorthogonal GPCR 标记。

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化学 问题 134 非规范氨基酸 (ncAA) 琥珀停止密码子抑制 合并效率 正交氨基酰 tRNA 合成酶/tRNA 对 光交联映射 蛋白质结合界面 bioorthogonal 化学,荧光显微镜
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