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En enkel og effektiv metode til In Vivo hjerte-specifikke genmanipulation af Intramyocardial injektion i mus
Freezing, Thawing, and Packaging Cells for Transport
Journal (Biology)
Freezing, Thawing, and Packaging Cells for Transport
Horizontal Slice Preparation of the Retina
Journal (Biology)
Horizontal Slice Preparation of the Retina
 
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En enkel og effektiv metode til In Vivo hjerte-specifikke genmanipulation af Intramyocardial injektion i mus

Article DOI: 10.3791/57074-v • 06:42 min • April 16th, 2018
April 16th, 2018 •
*1,2, *2, *2, 1, 1, 1, 1,2
1Department of Cardiology, The First Affiliated Hospital of Bengbu Medical College, 2Department of Cardiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University
* These authors contributed equally

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Her præsenterer vi en protokol for hjerte-specifikke genmanipulation i mus. Under anæstesi, var musen hjerter externalized gennem den fjerde interkostale rum. Efterfølgende, blot adenovirus kodning specifikke gener blev sprøjtet med en sprøjte ind i myokardiet, efterfulgt af protein udtryk måling via i vivo billeddannelse og Western analyse.

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Biologi spørgsmål 134 medicin hjerte mus gen overekspression knockdown hjerte
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