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JoVE Journal Genetics
Chromatin Immunopräzipitation (ChIP) Histon-Modifikationen von Saccharomyces cerevisiae
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Chromatin Immunopräzipitation (ChIP) Histon-Modifikationen von Saccharomyces cerevisiae

Article DOI: 10.3791/57080
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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December 29th, 2017

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Hier beschreiben wir ein Protokoll für Chromatin Immunopräzipitation der geänderten Histone aus der angehende Hefe Saccharomyces Cerevisiae. Immunoprecipitated DNA wird anschließend für die quantitative PCR verwendet, um die Fülle und die Lokalisierung von Histon post-translationalen Modifikationen während des Genoms zu befragen.

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