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JoVE Journal Genetics
Imunoprecipitação da cromatina (ChIP) de modificações do Histone de Saccharomyces cerevisiae
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Imunoprecipitação da cromatina (ChIP) de modificações do Histone de Saccharomyces cerevisiae

Article DOI: 10.3791/57080
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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December 29th, 2017

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Aqui, descrevemos um protocolo para a imunoprecipitação da cromatina de histonas modificadas de brotamento levedura Saccharomyces cerevisiae. Immunoprecipitated DNA é usado posteriormente para PCR quantitativo para interrogar a abundância e a localização de modificações borne-translational do histone durante todo o genoma.

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