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JoVE Journal Biochemistry
Método para Refolding eficiente y la purificación del quimioreceptor ligando vinculante dominio
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Método para Refolding eficiente y la purificación del quimioreceptor ligando vinculante dominio

Article DOI: 10.3791/57092-v • 14:25 min • December 12th, 2017
December 12th, 2017 •
1,2, 1,2,3
1Infection and Immunity Program, Monash Biomedicine Discovery Institute, 2Department of Microbiology, Monash University, 3Department of Biochemistry and Molecular Biology, Monash University

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Se presenta un procedimiento para el refolding el dCACHE periplasmic dominio obligatorio del ligand del jejuni del Campylobacter quimioreceptor Tlp3 de cuerpos de inclusión y de la purificación para producir cantidades de miligramos de proteína.

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Bioquímica número 130 quimiotaxis quimioreceptor transductor-como la proteína dominio detección del ligando dCACHE refolding
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