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Immunology and Infection

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JoVE Journal Immunology and Infection
Ontleden multi signalering eiwitcomplexen door Bimolecular complementatie affiniteit zuivering (BiCAP)
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Ontleden multi signalering eiwitcomplexen door Bimolecular complementatie affiniteit zuivering (BiCAP)

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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Dit manuscript beschrijft het protocol voor Bimolecular complementatie affiniteit zuivering (BiCAP). Deze nieuwe methode vergemakkelijkt de specifieke isolatie en downstream proteomic karakterisering van elk twee interacterende eiwitten, terwijl met uitzondering van VN-complexvorm individuele eiwitten evenals complexen gevormd met concurrerende bindende partners.

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Immunologie en infecties probleem 136 eiwit-eiwitinteractie Proteomics Interactomics BiCAP signaaltransductie Bimolecular fluorescentie complementatie Nanobody
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