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Immunology and Infection

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JoVE Journal Immunology and Infection
Dissection multi-signalisation Complexes protéiques par Purification d’affinité de complémentation bimoléculaire (BiCAP)
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Dissection multi-signalisation Complexes protéiques par Purification d’affinité de complémentation bimoléculaire (BiCAP)

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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Cet article décrit le protocole pour la Purification d’affinité de complémentation bimoléculaire (BiCAP). Cette nouvelle méthode facilite l’isolement spécifique et la caractérisation de la protéomique en aval de toute deux protéines qui interagissent, tandis que hors ONU complexée protéines individuelles ainsi que des complexes formés avec des partenaires de liaison concurrentes.

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Immunologie et Infection numéro 136 interactions protéine-protéine protéomique interactomique BiCAP transduction du Signal la complémentation de fluorescence bimoléculaire Nanobody
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