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Journal / Immunology and Infection / 解離性個体の親和性の浄化 (BiCAP)…
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Immunology and Infection

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JoVE Journal Immunology and Infection
解離性個体の親和性の浄化 (BiCAP) によって多蛋白質シグナリング複合体
Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
Journal (Immunology and Infection)
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Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts
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解離性個体の親和性の浄化 (BiCAP) によって多蛋白質シグナリング複合体

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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本稿では、二分子の相補性の親和性の浄化 (BiCAP) のプロトコルについて説明します。この手法は競合結合パートナーで形成された複合体と同様、国連複合体の個々 のタンパク質を除く、特定の分離と任意の 2 つの相互作用の蛋白質の下流のプロテオーム解析を促進します。

Tags

免疫学、感染症、問題 136、プロテオミクス、Interactomics、BiCAP、シグナル伝達蛋白質蛋白質の相互作用、分子蛍光補完、生物学: ナノボディで
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