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Immunology and Infection

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JoVE Journal Immunology and Infection
Dissekere multi protein Signaling komplekser af Bimolecular komplementering affinitet rensning (BiCAP)
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Dissekere multi protein Signaling komplekser af Bimolecular komplementering affinitet rensning (BiCAP)

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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Dette manuskript beskriver protokollen for Bimolecular komplementering affinitet rensning (BiCAP). Denne nye metode letter specifikke isolering og downstream proteom karakterisering af enhver to interagerende proteiner, mens bortset fra FN-kompleksbundet individuelle proteiner samt komplekser dannet med konkurrerende bindende partnere.

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Immunologi og infektion sag 136 Protein-protein interaktioner proteomik Interactomics BiCAP og signaltransduktion Bimolecular fluorescens komplementering Nanobody
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