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Journal / Immunology and Infection / Пройдя через сигнализации различных белковых…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Пройдя через сигнализации различных белковых комплексов, очищение сродства Bimolecular дополнения (BiCAP)
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Пройдя через сигнализации различных белковых комплексов, очищение сродства Bimolecular дополнения (BiCAP)

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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Эта рукопись описывает протокол для Bimolecular очищение сродства комплементарности (BiCAP). Этот новый метод облегчает конкретные изоляции и вниз по течению proteomic характеристик любых двух взаимодействующих белков, хотя исключая ООН complexed индивидуальных белков, а также комплексы с конкурирующими привязки партнерами.

Tags

Иммунология и инфекции выпуск 136 белок белковых взаимодействий протеомики Interactomics BiCAP трансдукции сигнала Bimolecular флуоресценции комплементарности наночастицы
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