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Journal / Immunology and Infection / Generatie van monoklonale antistoffen tegen…
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Immunology and Infection
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Generatie van monoklonale antistoffen tegen natuurlijke producten
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Generatie van monoklonale antistoffen tegen natuurlijke producten

Article DOI: 10.3791/57116-v • 12:15 min • April 6th, 2019
April 6th, 2019 •
1, 2, 3, 4, 3, 3, 5
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

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Dit artikel bevat een gedetailleerd protocol voor de voorbereiding en de evaluatie van monoklonale antistoffen tegen natuurlijke producten voor gebruik in verschillende immunoassay. Deze procedure omvat immunisatie, celfusie, indirecte competitieve ELISA voor positieve kloon screening en monoclonal hybridoma voorbereiding. De specificaties voor de karakterisering van het antilichaam met behulp van MALDI-TOF-MS en ELISA analyses zijn ook beschikbaar.

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Immunologie en infecties probleem 146 monoklonaal antilichaam kunstmatige antigeen natuur product kleine moleculaire compound immunisatie hybridoma
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