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Journal / Immunology and Infection / Generation af monoklonale antistoffer mod…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation af monoklonale antistoffer mod naturprodukter
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Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry
Journal (Immunology and Infection)
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Generation af monoklonale antistoffer mod naturprodukter

Article DOI: 10.3791/57116-v • 12:15 min • April 6th, 2019
April 6th, 2019 •
1, 2, 3, 4, 3, 3, 5
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

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Denne artikel giver en detaljeret protokol for forberedelse og evaluering af monoklonale antistoffer mod naturlige produkter til brug i forskellige immunassays. Denne procedure omfatter vaccination, cellefusion, indirekte konkurrerende ELISA for positive klon screening og monoklonale hybridom forberedelse. Specifikationer for antistof karakterisering ved hjælp af MALDI-TOF-MS og ELISA analyser er også tilgængelige.

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Immunologi og infektion spørgsmålet 146 monoklonalt antistof kunstige antigen natur produkt små molekylære sammensatte immunisering hybridom
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